Enzymatic Hydrolysis and Bioethanol Production from Neem Tree Leaves (AzadirachtaIindica)

Authors

  • Yusuf, M. National Food and Drugs Administration and Control Agency, Nigeria
  • Tambuwal, A.D. Usmanu Danfodiyo University, Sokoto
  • Hadi, B. A. Shehu Shagari College of Education, Sokoto
  • Tukur, A. Federal University Dutse, Jigawa

Keywords:

Azadirachtaindica, Bacillus firmus, Bio-ethano, Enzymatic Hydrolysis, Ligno-cellulosic Biomass

Abstract

Bio-ethanol was produced from Neems tree leaves (Azadirachta indica) through enzymatic hydrolysis using Bacillus firmus as fermenting agent. Dried powdered of Neem tree leaves (Azadirachta indica) was hydrolyzed using 1.0cm3, 1.5cm3 and 2.0cm3 of Bacillus suspension. After that the hydrolyzed samples were fermented using 3cm3 suspension of cultured bacteria (Bacillus firmus). After that, the fermented broths formed were distillated to obtained bioethanol. Acidified K2Cr2O7 was used to determine the concentration of the bioethanol produced. In which the absorbance of the bioethanol obtained using UV-Visible Spectrophotometer was extrapolated with the series concentration prepared with standard glucose solution. The FTIR Spectroscopy analysis of bio-ethanol produced confirms the presence of alcohol functional group present in the sample. The percentage yield of the bioethanol from the leaves using 1.0cm3, 1.5cm3 and 2.0cm3 of Bacillus suspension were 1.42%, 1.85%, and 1.72% yield respectively. The result showed that the Neem tree leaves that are from lignocelluloses materials can produce some appreciable percentage of bio-ethanol. Among the samples 1.5cm3of Bacillus suspension has shown the highest yield of 1.85%.         

Author Biographies

Yusuf, M., National Food and Drugs Administration and Control Agency, Nigeria

National Food and Drugs Administration and Control Agency, Nigeria

Tambuwal, A.D., Usmanu Danfodiyo University, Sokoto

Sokoto Energy Research Centre, Usmanu Danfodiyo University, Sokoto

Hadi, B. A., Shehu Shagari College of Education, Sokoto

Department of Chemistry, Shehu Shagari College of Education, Sokoto Nigeria

Tukur, A., Federal University Dutse, Jigawa

Department of Chemistry, Federal University Dutse, Jigawa State Nigeria

Downloads

Published

2016-06-25

How to Cite

Yusuf, M., Tambuwal, A.D., Hadi, B. A., & Tukur, A. (2016). Enzymatic Hydrolysis and Bioethanol Production from Neem Tree Leaves (AzadirachtaIindica). International Journal of Science for Global Sustainability, 2(2), 8. Retrieved from https://www.fugus-ijsgs.com.ng/index.php/ijsgs/article/view/250